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Raúl Padrón ORCID ID 0000-0002-1412-2450  |
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Publications
PDFs provided only for
educational purposes and colleagues research use
.
| Year |
Authors |
Title |
Reference |
Abstract | |
| 2020 |
Padrón, R., Ma, W., Duno-Miranda, S., Koubassova, N., Lee, K. H., Pinto, A., Alamo, L., Bolaños, P., Tsaturyan, A., Irving, T. and Craig, R. | The myosin interacting-heads motif present in live tarantula muscle explains tetanic and post-tetanic phosphorylation mechanisms. | |||
| 2020 |
Toepfer, C., Garfinkel, A., Venturini, G., Wakimoto, H., Repetti, G., Alamo, L., Sharma, A., Agarwal, R., Ewoldt, J., Cloonan, P., Letendre, J., Lun, M., Olivotto, I., Colan, S., Ashley, E., Jacoby, D., Michels, M., Redwood, C., Watkins, H., Day, S., Staples, J., Padrón, R., Chopra, A., Ho, C., Chen, C., Pereira, A., Seidman, J., Seidman, C. E. | Myosin sequestration regulates sarcomere function, cardiomyocyte energetics, and metabolism, informing the pathogenesis of hypertrophic cardiomyopathy | Circulation 2020; 141:828–842. DOI: 10.1161/CIRCULATIONAHA.119.042339. | ||
| 2020 |
Sulbaran, G, Biasutto, A., Mendez, F., Pinto, A., Alamo, A. and Padrón, R. | 18O labeling on Ser45 but not on Ser35 supports the cooperative phosphorylation mechanism on tarantula thick filament activation. | Biochem Biophys Res Comm. 524: 198-204. | ||
| 2020 |
L.
Alamo, A. Pinto, G. Sulbarán, J. Mavárez
& R. Padrón |
Lessons from a tarantula: New insights into myosin interacting-heads motif evolution and its implications on disease | Biophys. Rev https://doi.org/10.1007/s12551-017-0292-4. | ||
| 2018 |
K.
H. Lee, G. Sulbarán, S. Yang, J. Y. Mun, L.
Alamo, A.
Pinto, O. Sato, M. Ikebe, X. Liu, E. D. Korn, F.
Sarsoza,
S. I. Bernstein, R. Padrón & R. Craig
|
Interacting-heads motif has been conserved as a mechanism of myosin II inhibition since before the origin of animals | doi.org/10.1073/pnas.1715247115 | PDF
|
SI PDF |
| 2017 |
L.
Alamo, N. Koubassova, A. Pinto, R. Gillilan, A. Tsaturyan &
R. Padrón |
Lessons
from a tarantula: New insights into muscle thick filament and myosin
interacting-heads motif structure and function |
Biophys.
Rev. 9: 461-480 DOI 10.1007/s2551-017-0295-4 |
http://rdcu.be/vz7T |
Abstract |
| 2017 |
L. Alamo, J. S. Ware, A. Pinto, R. E. Gillilan, J. G. Seidman, C. E. Seidman & Raúl Padrón | Effects of myosin variants on interacting-heads motif explain distinct hypertrophic and dilated cardiomyopathy phenotypes | eLife
2017;6:e24634 doi: 10.7554/eLife.24634 |
PDF SI PDF |
Abstract |
| 2016 |
L.
Alamo, D. Qi, W. Wriggers, A. Pinto, J. Zhu, A. Bilbao, Richard E.
Gillilan, S. Hu, R.& R. Padrón. |
Conserved
Intramolecular Interactions Maintain Myosin Interacting-Heads Motifs
Explaining Tarantula Muscle Super-Relaxed State Structural Basis |
J.
Mol. Biol. 428: 1142-1164, 2016 |
PDF SI PDF |
Abstract |
| 2015 |
Sulbarán,
G., Alamo, L., Pinto, A., Márquez, G., Méndez, F.,
Padrón, R. & Craig, R. |
An
invertebrate smooth muscle with striated muscle myosin filaments |
Proc.
Nat. Acad. Sci. (USA) 112(42): E5660-E5668, 2015 |
PDF |
Abstract |
| 2015 |
Alamo,
L., Li, X., Espinoza-Fonseca, M., Pinto, A., Thomas, D., Lehman, W.
& Padrón, R. |
Tarantula
myosin free head regulatory light chain phosphorylation stiffens
N-terminal extension releasing it and blocking its docking back |
Mol.
BioSyst. 11: 2180-2189, 2015 |
PDF |
Abstract |
| 2015 |
Espinoza-Fonseca,
M., Alamo, L., Pinto, A., Thomas, D. & Padrón R. |
Sequential
Myosin Phosphorylñation Activates Tarantula Thick Filament via a
Disorder-Order Transition. |
Mol.
BioSyst.11: 2167-2179, 2015 |
PDF |
Abstract |
| 2014 |
Marquez,
G., Pinto, A., Alamo, L-, Baumann, B., Le, F., Winkler, H.,
Taylor, K. & Padrón R. |
A
method for 3D-reconstruction of a muscle thick filament using tilt
series images of a single filament electron tomogram. |
J.
Struct. Biol. 186: 265-272, 2014. |
PDF |
Abstract |
| 2013 |
Padrón,
R. |
Two
and a half years at the LMB that imprinted my scientific career
(1980-83) |
Chapter
38, in book “Memoirs and Consequences” (Editor Hugh Huxley), Published
by MRC Laboratory of Molecular Biology, Cambridge, U.K. |
PDF |
Abstract |
| 2013 |
G.
Sulbarán, A. Biasutto, L. Alamo, C. Riggs, A. Pinto, F. Mendez,
R. Craig and R. Padrón. |
Different
head environments in tarantula thick filaments support a cooperative
activation process. |
Biophys.
J. 105: 2114-2122, 2013 |
PDF |
Abstract |
| 2013 |
Rodriguez,
R., Soto, R., Lacruz, A., Rivas, Y., Flores, I., Falcone, M., Alamo, L.
y Padron, R. |
Leucina
en la proteína de unión de miosina cardiaca (MyBP-C)
encontrada en un paciente con miocardiopatía hipertrofica en
Venezuela. |
Interciencia: 38:
657-663, 2013. |
PDF |
Abstract |
| 2012 |
Pinto,
A., Sanchez, F., Alamo, L. & Padrón, R. |
The myosin interacting-heads motif is present in the relaxed thick filament of the striated muscle of scorpion | J.
Struct. Biol. 180: 469-478, 2012 |
PDF |
Abstract |
| 2011 |
Brito, R., Alamo, L.,
Lundberg, L, Guerrero, J.R., Pinto, A., Sulbarán, G.,
Gawinowicz, M. A., Craig, R. & Padrón, R.
|
molecular
model of phosphorylation-based activation and potentiation of
tarantula muscle thick filaments |
J.
Mol. Biol. 414: 44-61, 2011. |
PDF |
Abstract |
| 2011 |
Luther,
P., Winkler, H., Taylor, K., Zoghbi, M., Craig, R., Padrón, R.,
Squire, J. & Liu, J |
Direct
visualisation of myosin binding protein C bridging myosin and actin
filaments in intact muscle |
Proc. Nat. Acad.
Sci. (USA): 108(28):11423-8, 2011 |
PDF |
Abtract |
| 2011 |
Rodriguez
, R. & Padrón, R. |
Uso
de la genética en el diagnóstico de la
miocardiopatía hipertrófica familiar |
Avances Cardiol 31(1): 45 - 47, 2011 | PDF |
|
| 2011 |
Wriggers,
W., Alamo, R. & Padrón R. |
Matching
Structural Densities from Different Biophysical Origins with Gain and
Bias |
J. Struct. Biol.: 173: 445-450, 2011 | PDF |
Abstract |
| 2008 |
Alamo,
L., Wriggers, W., Pinto, A., Bártoli, F., Salazar, L., Zhao, F.,
Craig, R. & Padrón, R. |
Three-dimensional
Reconstruction of Tarantula Myosin Filaments Suggests How
Phosphorylation May Regulate Myosin Activity |
J.
Mol. Biol. 384: 780-797, 2008 |
PDF |
Abstract |
| 2008 |
Luther,
P. K., Knupp, C., Craig, R., Padrón, R., Harris, S., Patel,
J., Moss, R. L. & Bennet, P. M. |
Understanding the organization and role of myosin binding protein C in striated muscle by analysis of normal and MyBPC knockout muscle. | J. Mol. Biol. 384: 60-72, 2008 | PDF |
Abstract |
| 2008 |
F. Q. Zhao, R. Padrón & R. Craig. | Blebbistatin stabilizes the helical order of myosin filaments by promoting the switch 2 closed state. | Biophys. J. 95:
3322-3329, 2008 |
PDF |
Abstract |
| 2008 |
Padrón,
R. |
El mecanismo molecular de la regulación de la contracción muscular. | Arch. Ven. Farm Ter. 27(1): 2-4, 2008. | PDF |
Abstract |
| 2007 |
Padrón,
R. |
Filamento
de miosina: modelo atómico |
Investigación
y Ciencia 369: 28-30, 2007 (Spanish version of Scientific American) |
PDF |
Abstract |
| 2006 |
Padrón,
R. & Alamo, L.  |
Review:
The use of negative staining and cryo-electron microscopy to understand
the molecular mechanism of myosin-linked regulation of striated muscle
contraction. |
Acta
Microsc. 13: 14-29, 2004. (actually published in 2006!) |
Abstract | |
| 2005 |
Woodhead,
J., Zhao, F., Craig, R., Egelman, E., Alamo, L. & Padrón, R. |
Atomic model of a myosin filament in the relaxed state. | Nature 436: 1195-1199 , 2005. | PDF |
Abstract Supplementary information |
| 2004 |
Zoghbi,
M. E., Woodhead,J., Craig, R. & Padrón, R. |
Helical order in tarantula thick filaments requires the closed conformation of the myosin head. | J. Mol. Biol. 342: 1223-1236, 2004. | PDF |
Abstract |
| 2004 |
Craig, R. & Padrón, R. | Molecular Structure of the Sarcomere | Chapter
7. text book “Myology” (Edit. A. G. Engel y C. Franzini-Armstrong), 3th edition. McGraw-Hill, Inc., New York. 2004. (invited review) |
PDF |
Abstract |
| 2004 |
Ramírez,
C. D. & Padrón R. |
Cardiomiopatía hipertrófica familiar: genes, mutaciones y modelos animales. | Inv. Clin. 45(1): 69-100, 2004. | PDF HTML (very slow link!) |
Abstract |
| 2003 |
Luther,
P.K. , Padrón, R., Ritter, S, Craig, R. & Squire, J. M.  |
Heterogeneity of Z-band structure within a single muscle sarcomere: Implications for sarcomere assembly. | J. Mol. Biol. 332: 161-169, 2003. | Abstract | |
| 2001 |
Hidalgo,
C., Padrón, R., Horowitz, R., Zhao, F. & Craig, R. |
Purification of native myosin filaments from muscle. | Biophys. J. 81: 2817-2826, 2001. | Abstract | |
| 2001 |
Hidalgo,
C., Craig, R., Ikebe, M. & Padrón, R. |
Mechanism of phosphorylation of the regulatory light chain of myosin from tarantula striated muscle. | J. Muscle Res. Cell Motil. 22. 51-59, 2001. | Abstract | |
| 2000 |
Offer,
G.; Knight, P.; Burgess, S. A. Alamo, L. & Padrón, R. |
A new model for the surface arrangement of myosin molecules in heads in tarantula thick filaments. | J. Mol. Biol. 298: 239-260, 2000. | Abstract | |
| 1998 |
Padrón,
R.; Alamo, L.; Murgich, J. & Craig, R.  |
Towards an atomic model of the thick filament of muscle. | J. Mol. Biol. 275: 35-41, 1998. | Abstract | |
| 1997 |
Mateu,
L.; Moran, O.; Padrón, R., Borgo, M., Vonasek, E., Marquez,
V.& Luzzati, V. |
The action of local anesthetics on myelin structure and nerve conduction in toad sciatic nerve. | Biophys. J.: 70: 2581-2587, 1997. | PDF |
Abstract |
| 1997 |
San-Blas,
G.; Padrón, R.; Alamo, L. & San-Blas, F. |
Use of morphology index histograms to quantify populations of the fungal pathogenParacoccidioides brasiliensis. | Microbiology 143: 197-202, 1997. | Abstract | |
| 1996 |
Caputo,
C. & Padrón, R. |
Mecanismo de la contracción muscular | Cap.
26. Pp 610-647 Biofísica y Fisiología Celular (eds. Bezanilla, F., Latorre, R., López Barneo, J. y Llinás, R.) Universidad de Sevilla, Sevilla, España. 1996. |
PDF |
Abstract |
| 1995 |
Padrón,
R., Alamo, L., Guerrero, J. R., Granados, M., Uman, P. & Craig, R.  |
Three-dimensional reconstruction of thick filaments from rapidly frozen, freeze-substituted tarantula muscle. | J.
Struct. Biol.: 115(3): 250-257, 1995. |
Abstract | |
| 1993 |
Padrón,
R., Rodriguez, J.; Guerrero, J. R. & Alamo, L. |
Fraying of thick filaments from tarantula muscle into subfilaments. | Acta Microsc. 2(1): 85-92, 1993. | PDF |
Abstract |
| 1993 |
Padrón,
R., Guerrero, J. R., Alamo, L., Granados, M., Gherbesi, N. & Craig,
R. |
Direct visualization of myosin filament symmetry in tarantula striated muscle by electron microscopy. | J. Struct. Biol.: 111: 17-21, 1993. | Abstract |
|
| 1992 |
Craig,
R., Alamo, L. & Padrón, R. |
Structure of the myosin filaments of relaxed and rigor striated vertebrate muscle studied by rapid freezing electron microscopy. | J. Mol. Biol. 228: 474-487, 1992. | PDF |
Abstract |
| 1992 |
Guerrero,
J. R. & Padrón, R. |
The Substructure of the backbone of the thick filament from Tarantula muscle. | Acta Microsc. 1(2): 63-83, 1992. | PDF |
|
| 1992 |
Padrón,
R.; Granados, M.; Alamo, L.; Guerrero, J. R. & Craig, R. |
Visualization of myosin helices in sections of rapidly frozen relaxed tarantula muscle. | J. Struct. Biol. 108(3): 269-276, 1992. | PDF |
Abstract |
| 1991 |
Alamo,
L., Padrón, R., Craig, R. & Hidalgo, C. |
Método para la determinación directa de la simetría rotacional de filamentos gruesos de músculo por procesamiento digital de imágenes. | Acta Cient. Ven. 42(2): 59-63, 1991. | PDF |
Abstract |
| 1991 |
Craig,
R., Padrón, R. & Alamo, L. |
Direct determination of myosin filament symmetry in scallop striated adductor muscle by rapid freezing and freeze-substitution. | J. Mol. Biol. 220: 125-132,1991. | PDF |
Abstract |
| 1991 |
Padrón,
R., Panté, N., Sosa, H. & Kendrick-Jones, J. |
X-ray diffraction study of the structural changes accompanying phosphorylation of tarantula muscle. | J. Muscle Res. Cell
Motil. 12(3): 235-241, 1991. |
PDF |
Abstract |
| 1989 |
Padrón, R. & Craig, R. | Disorder induced in non-overlap myosin crossbridges by loss of adenosine triphosphate. | Biophys. J. 56: 927-933, 1989. | PDF |
Abstract |
| 1988 |
Panté,
N., Sosa, H. & Padrón, R. |
Estudio por difracion de rayos-X de los cambios estructurales que acompañan la fosforilación de los filamentos gruesos de músculo de tarántula. | Acta Cient. Ven. 39(3): 230-236, 1988. | Abstract | |
| 1988 |
Padrón,
R., Alamo, L. Craig, R. & Caputo, C. |
A method for quick-freezing live muscles at known instants during contraction with simultaneous recording of mechanical tension. | J. Microsc. (Oxford) 151: 81-102, 1988. | PDF |
Abstract |
| 1988 |
Sosa,
H., Panté, N. & Padrón, R.  |
Análisis de la sección ecuatorial del patrón de difracción de rayos-X de músculos estriados de tarántula en diferentes condiciones experimentales. | Acta Cient. Ven. 39(1): 51-59, 1988. | Abstract | |
| 1987 |
Alamo,
L., Padrón, R., Craig, R. & Caputo, C. |
Método para el congelamiento rápido con nitrógeno o helio líquido de músculos en contracción tetánica. | Acta Cient. Ven. 38(4): 513-514, 1987. | PDF |
Abstract |
| 1987 |
Martelo,
M.J. & Padrón, R. |
Método rápido para el aislamiento y purificación de miosina de músculo de tarantula. | Acta Cient. Ven. 38(3): 394-395, 1987. | PDF |
Abstract |
| 1987 |
Padrón,
R., Queralt, V. & Sosa, H. |
Método para la cuantificación de cambios en la capacidad de retención de agua en carne utilizando difracción de rayos-X. | Acta Cient. Ven. 38(2): 287-288, 1987. | PDF |
Abstract |
| 1987 |
Craig,
R., Padrón, R. & Kendrick-Jones: |
Structural changes accompanying phosphorylation of tarantula muscle thick filaments. | J. Cell. Biol. 105: 1319-1327, 1987. | Abstract |
|
| 1986 |
Sosa,
H., Panté, N., & Padrón, R. |
Determinación de la sección ecuatorial del patrón de difracción de rayos-X de músculo vivo de tarántula. | Acta Cient. Ven. 37(5): 587-588, 1986. | Abstract | |
| 1986 |
Panté,
N., Sosa, H.& Padrón, R. |
Predicción de cambios relativos en el patrón ecuatorial de difracción de rayos-X de músculo estriado causados por la activación de la contracción muscular. | Acta Cient. Ven. 37: 223-225, 1986. | Abstract | |
| 1985 |
Crowther,
R.A.; Padrón, R. & Craig, R.: |
The arrangement of the heads of myosin in relaxed thick filaments from Tarantula muscle. | J. Mol. Biol. 184(3): 429-439, 1985. | Abstract |
|
| 1984 |
Padrón,
R. & Huxley, H.E. |
The effect of the ATP analogue AMPPNP on the structure of crossbridges in vertebrate skeletal muscle: X-ray diffraction and mechanical studies. | J. Muscle Res. Cell. Motil 5(6): 613-655, 1984. | PDF |
Abstract |
| 1984 |
Egelman,
E.H. & Padrón, R. |
X-ray diffraction evidence that actin is a 100 Å filament. | Nature, 307: 56-58, 1984. | Abstract |
|
| 1982 |
Padrón,
R. & Mateu, L. |
Repetitive propagation of action potentials destabilizes the structure of the myelin sheath: a dynamic X-ray diffraction study. | Biophys. J. 39: 183-188, 1982. | PDF |
Abstract |
| 1981 |
Padrón,
R. & Mateu, L. |
Alteración reversible de la estructura de las membranas de mielina de nervio ciático de rana por comprensión mecánica in vivo: estudio por difracción de rayos-X. | Acta Cient. Venez. 32(2): 166-171, 1981. | PDF |
Abstract |
| 1981 |
Padrón,
R. & Mateu, L. |
In vivo structure of frog sciatic nerve myelin membranes: a X-ray diffraction study at 13 Å resolution. | J. Neurosc. Res. 6(2): 251-260, 1981. | PDF |
Abstract |
| 1980 |
Padrón,
R. & Mateu, L. |
The effect of repetitive propagation of action potentials on the structure of toad sciatic nerve myelin membranes: a X-ray diffraction study at 11 Å resolution. | J. Neurosc. Res. 5(6): 611-620, 1980. | PDF |
Abstract |
| 1980 |
Padrón,
R., Salas, R. & Mateu, L. |
La estructura de las membranas de mielina de nervio ciático de rana: determinación in vivo por difracción de rayos-X a 15 Å de resolución espacial. | Acta Cient. Venez. 31(3): 224-228, 1980. | PDF |
Abstract |
| 1980 |
Padrón,
R. & Caputo, C. |
Canonical equivalent circuits of isolated barnacle muscle fibers. | Acta Cient. Venez. 31(2): 160-166, 1980. | PDF |
Abstract |
| 1980 |
Padrón,
R., Mateu, L. & Requena, J. |
A dynamic X-ray diffraction study of anesthesia action: Changes in myelin structure and electrical activity recorded simultaneously from frog sciatic nerves treated with n-alkanes. | Biochim. Biophyhs. Acta 602: 221-233, 1980. | PDF |
Abstract |
| 1979 |
Padrón,
R., Mateu, L. & Kirschner, D.A. |
X-ray diffraction study of the kinetics of myelin lattice swelling: the effect of divalent cations. | Biophys. J. 28: 231-239, 1979. | PDF |
Abstract |
| 1979 |
Padrón, R., Requena, J. & Mateu, L. | A dynamic X-ray diffraction study of anesthesia action: Thickening of the myelin membrane by n-pentane. | Biochim.
Biophys. Acta 552: 535-539, 1979. |
PDF |
Abstract |
| 1979 |
Padrón,
R. |
Alteración de la estabilidad estructural de la mielina: Estudio por difracción de rayos-X. | Tesis de Grado para optar al Título de Ph.Sc. en Biología, mención Fisiología y Biofísica. Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Estudios Avanzados (CEA), 1979. | PDF |
Abstract |
| 1977 |
Mateu,
L., Kirchhausen, T., Padrón, R. & Camejo, G. |
Small-angle X-ray scattering study of human serum low density lipoproteins with differential reactivity for an arterial proteoglycan. | J. Supramol. Struct. 7: 435-442, 1977. | PDF |
Abstract |
| 1977 |
Padrón,
R. |
La estabilidad estructural de la mielina: Estudio por difracción de rayos-X. | Trabajo de Grado para optar al Título de M.Sc. en Biología, mención Fisiología y Biofísica. Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Estudios Avanzados (CEA), 1977. | PDF |
Abstract |
| 1976 |
Camejo,
G., Mateu, L., Lalaguna, F., Padrón, R., Waich, A., Aquatella,
H. & Vega, H. |
Structural individuality of human serum LDL associated with a differential affinity for a macromolecular component of the arterial wall. | Artery 2(1): 79-97, 1976. | PDF |
Abstract |
| 1974 |
Padrón,
R. |
Propiedades eléctricas pasivas de fibras musculares aisladas de Balanus. | Trabajo Especial para optar al Título de Ingeniero Eléctrico. Universidad Central de Venezuela (UCV), Facultad de Ingeniería, 1974. | PDF |
Abstract |
